Results : the primary cells formed a monolayer in 18th day . von - kossa staining presented mineral deposition after the cells had been cultured 30 days in calcified solution . conclusion : bmsc can be isolated and cultured easily 结果:原代细胞培养第18d细胞融合成单层，长满整个培养皿;倒置显微镜下观察，矿化条件下，细胞培养30天， von一kassa染色显示有局灶状钙盐沉积。
